Poster Presentation Australian Society for Fish Biology Conference 2017

Taking estuarine eastern blue spot goby (Psedudogobius sp.) fish embryo testing (FET) out of infancy. (#153)

W. Mehler 1 2 , Jessica French 1 2 , Mick Keough 2 , Vincent Pettigrove 1 2 , Steve Swearer 2 , Nicole Barbee 2 , Kathryn Hassell 1 2
  1. Centre for Aquatic Pollution Identification and Management (CAPIM), School of Biosciences, The University of Melbourne, Parkville, Victoria, Australia
  2. The University of Melbourne, Parkville, VIC, Australia

The use of fish embryo testing (FET) is strongly being encouraged to replace acute and chronic toxicity testing with juvenile fish. Much of the work that has been conducted using FET has been to evaluate chemical safety and effluents with guidance proposed and developed by OECD in 2006 and ISO in 2007. The current literature typically uses one of three model freshwater fish species, zebrafish (Danio rerio), the Japanese medaka (Oryzias latipes) or the fathead minnow (Pimephales promelas).  These species are commonly used since information pertaining to physiology, embryonic development, and sensitivity are abundant. However, none of these fishes occur naturally in Australia, and therefore lack environmental realism (in an Australian context).  Therefore, for Australian environmental monitoring and ecotoxicological research there is a need to establish equivalent tests in locally relevant species (freshwater, estuarine and marine).  This presentation will showcase some of the fish embryo endpoints currently being assessed (heartbeat/min, cumulative hatch rates, survival, size at hatch) in the eastern blue spot goby (Pseudogobius sp.) a small, estuarine species native to south-eastern Australia.  Specifically, this presentation will discuss some of the strengths and limitations of this embryo assay as it pertains to culturing, testing setup and methodology (for both water and sediments) as well as the species sensitivity and environmental realism/relevance.